FRAP of GFP-tagged Nrg in epithelial cells of live embryos. Side views of epithelial cells in wild-type (A), nrx IV (B), cont (C) and Dfvari (D) embryos at stage15. Pre-bleach images are shown on the left. The sections of cell interface that were bleached are indicated with boxes. Images on the right show the recovery of fluorescence 160 seconds after bleaching. Bar: 5 μm. Schematic representation of the distribution of septate junction components, Nrg, Nrx IV and Cont in each genotype.
Laval et al. BMC Cell Biology 2008 9:38 doi:10.1186/1471-2121-9-38