Open Access Highly Accessed Research article

The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response

Mattias K Andersson1*, Anders Ståhlberg23, Yvonne Arvidsson1, Anita Olofsson1, Henrik Semb3, Göran Stenman1, Ola Nilsson1 and Pierre Åman1

Author Affiliations

1 Lundberg Laboratory for Cancer Research, Department of Pathology, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden

2 Center for Brain Repair and Rehabilitation (CBR), Department of Clinical Neuroscience and Rehabilitation, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden

3 Stem Cell Center, Lund University, Lund, Sweden

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BMC Cell Biology 2008, 9:37  doi:10.1186/1471-2121-9-37

Published: 11 July 2008

Additional files

Additional file 1:

Immunofluorescence and transfections. Immunostaining and transfections of FET proteins. (a) Endogenous and transient FET protein expression in four different cultured cell types with DAPI staining of nuclei (blue). Scale bars indicate 10 μM. (b) Stable expression of FET-EGFP proteins in HT1080 with DAPI staining (blue). Scale bars indicate 10 μM.

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Additional file 2:

Cell area measurements. Clones of stable transfectants were seeded out, stained and imaged as described in Materials and methods. Average cell areas were calculated from 130–200 cells in five images per clone. Error bars show standard error of mean.

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Additional file 3:

Proliferation assay. Western blot showing FET expression in contact-inhibited and actively proliferating F470 cells as well as in serum stimulated and starved HT1080 cells. Beta actin expression is used as a loading control.

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Additional file 4:

Primary antibodies. Primary antibodies used.

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Additional file 5:

Primer sequences. Primers used for cDNA cloning and quantitative real-time PCR.

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