Glutathione status in RAW 264.7 cells incubated with CEES/LPS. Macrophages were incubated with 50 ng/ml LPS or/and 500 μM CEES for 12 h. Total GSH (GSH+GSSG) and GSSG levels were measured using a GSH assay kit (see Materials and Methods section) in cell lysates and normalized to total protein. Numbers show the percentage of GSSG in total GSH. Mean values not sharing a common letter are significantly different (p < 0.05).
Paromov et al. BMC Cell Biology 2008 9:33 doi:10.1186/1471-2121-9-33