Tat affects rRNA processing. (A): scheme of Drosophila rRNA precursor with cleavage sites corresponding to the two alternative processing pathways, α and β. Sequences of cleavages generating the two different pathways are highlighted under the scheme (see reference 29 for detail). Solid bars show probe positions. (B-D): Northern blot of RNA extract from control (WG) and transgenic strains (Tat-strain) with (+) or without (-) heat shock treatment. Probe I, derived from the ITS1 region and closed to cleavage site 2, was used in B. The same blot was hybridized using respectively probe II (C) derived from the ITS1 region including cleavage site 3 and probe III (D) derived from the ETS region, closed to cleavage site 1. The different immature products are indicated on the left (see scheme 4A). Tubulin is shown as loading control. Histograms represent the average from three independent experiments.
Ponti et al. BMC Cell Biology 2008 9:32 doi:10.1186/1471-2121-9-32