Transient transfection of mLTC-1 cells with mutant construct of the FasL promoter. Cultured mLTC-1 cells transfected with FasL-P272 or FasL-P272m were treated with 100 nM CORT for 12 h followed by luciferase activity assays. The transfected cells which were treated with vehicle served as controls. The mLTC-1 cells with CORT treatment dramatically increased transcriptional activity of FasL-P272, but not FasL-P272m. Up to 80% of the FasL promoter activity was lost in mLTC-1 cells transfected with the FasL-P272m when the cells were incubated with 100 nM CORT, compared with that in the cells transfected with FasL-P272. Asterisk indicates that the difference between FasL-P272 and FasL-P272m is statistically significant (P < 0.05).
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31