The enhancement of transcriptional activity of FasL promoter in CORT- treated mLTC-1 cells. A. Schematic representations of known transcription factor binding sites in the 5' regulatory region of the FasL gene and reporter-luciferase constructs used in these studies. Lengths of the promoter regions in the 5' truncation constructs are relative to the transcription start site. B. Analysis of transcriptional activity of FasL promoter in mLTC-1 cells subjected to CORT treatment. mLTC-1 cells were transiently transfected with luciferase reporter constructs containing the -618 to +71 bp region of FasL promoter (FasL-P689). The transfected cells were treated with vehicle as control or treated with 100 nM CORT for 12 h. The luciferase activity, normalized to an internal Renilla control, was compared with the activity of the transfected but no treated cells. There data are representative of at least three independent experiments. ** denotes a significant difference compared with control at P < 0.01.
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31