The enhancement of FasL mRNA and protein expression in CORT-treated mLTC-1 cells. A. Expression of FasL mRNA was determined by RT-PCR in mLTC-1 cells. Band intensity was normalized to the β-actin product generated using specific primers to β-actin. Lane 1. Marker; Lane 2. The level of FasL mRNA in mLTC-1 cells untreated with CORT; Lane 3. The level of FasL mRNA in mLTC-1 cells treated with 100 nM CORT for 12 h; Lane 4. The level of FasL mRNA in mLTC-1 cells treated with 100 nM CORT plus 100 ng/ml CsA for 12 h. The level of FasL mRNA was increased following the CORT treatment. The mRNA level was decreased in mLTC-1 cells with CORT and CsA treatment. B. Expression of FasL protein in mLTC-1 cells was determined by Western blot. Lane 1, 2, 3 is without CORT treatment, with CORT treatment and CORT plus CsA, respectively. The expression of FasL was increased with CORT treatment and decreased with CORT plus CsA treatment.
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31