Expression of NFAT2 in Leydig cells. A. NFAT2 was determined by RT-PCR using specific primers. β-actin was used as a control to monitor RNA quality. Lane 1 is the marker. The DNA was visualized under UV immediately after staining with ethidium bromide. B. Western blotting was used to determine the expression of NFAT2 protein in Leydig cells. Lane 1. The lysates from primary rat Leydig cells; Lane 2. The lysates extracted from T cells as a positive control; Lane 3. The lysates from mLTC-1 cells. β-actin was used as a control to monitor protein quality. The proteins were resolved by SDS-PAGE and immunoblotted with anti-NFAT2. Strong bands of NFAT2 protein in mLTC-1 and primary rat Leydig cells can be seen, which is similar to that in T cells.
Chai et al. BMC Cell Biology 2008 9:31 doi:10.1186/1471-2121-9-31