Table 1

Proteins identified by tandem mass spectrometry.

(A) Proteins associated with DRMs

DRM proteins

References

Accession #

Score

Description


IPI00006211

175

VAMP (Vesicle-associated membrane protein)-associated protein B and Ca

[42]

IPI00008453

122

Coronin-1C

[26]

IPI00013981

95

YES tyrosine kinase

[25]

IPI00015148

131

Ras-related protein Rap-1b

[40]

IPI00018511

95

Tubulin beta-4q chain

[40]

IPI00022418

285

Splice Isoform 1 of Fibronectin precursor

[26]

IPI00027438

388

Flotillin-1

[16, 25, 39]

IPI00029625

133

Flotillin-2

[16, 29, 39, 40]

IPI00030910

185

GPI-anchored protein p137a

[43]

IPI00030919

95

Mitogen-activated protein kinase kinase 1 interacting protein 1

[25], [40]

IPI00030939

185

Alpha subunit of GsGTP binding protein

[25]

IPI00216308

2152

Voltage-dependent anion-selective channel protein 1

[44]

IPI00220416

101

Ubiquinol-cytochrome c reductase complex 14 kDa protein

[29, 44]

IPI00221232

106

G-protein gamma-12 subunit variant

[25]

IPI00334190

552

Stomatin-like protein 2

[44]

IPI00337415

81

Guanine nucleotide-binding protein G(i) alpha-1 subunit

[25]

IPI00339269

163

Heat shock 70 kDa protein 6

[40]

IPI00440493

440

ATP synthase alpha chain, mitochondrial precursor

[40]

IPI00554701

72

Ubiquinol-cytochrome c reductase complex 7.2 kDa protein

[29, 44]

IPI00554711

603

Junction plakoglobin

[25]

IPI00641181

143

MARCKS-like 1

[28]


(B) Nuclear proteins shown to exist in other subcellular locations

Nuclear proteins

Accession #

Score

Description

Evidence for shuttling


IPI00000690

87

Splice Isoform 1 of Programmed cell death protein 8, mitochondrial precursor

Yes: [46]

IPI00012048

76

Nucleoside diphosphate kinase A

Yes: [47]

IPI00012442

190

Ras-GTPase-activating protein binding protein 1

Yes: [48]

IPI00016249

91

Fragile X mental retardation syndrome-related protein 1

Yes: [49]

IPI00016250

71

Fragile X mental retardation syndrome-related protein 2

Yes: [49]

IPI00021840

230

40S ribosomal protein S6

Yes: [50]

IPI00023591

158

Transcriptional activator protein PUR-alpha

No:

IPI00024157

114

FK506-binding protein 3

No:

IPI00027096

74

39S ribosomal protein L19, mitochondrial precursor

Yes: UniProt 49406

IPI00027415

171

MLEL1 protein

No:

IPI00028376

92

Mitochondrial import inner membrane translocase subunit TIM8 A

Yes: [51]

IPI00032003

60

Emerin

No:

IPI00042578

83

Similar to heterogeneous nuclear ribonucleoprotein A1 isoform a

Yes: [58]

IPI00165506

125

Polymerase delta interacting protein 2

Yes: [52]

IPI00215637

131

DEAD-box protein 3, X-chromosomal

Yes: [53]

IPI00215780

167

40S ribosomal protein S19

Yes: [54]

IPI00217468

87

Histone H1.5

No:

IPI00219330

206

Splice Isoform 5 of Interleukin enhancer-binding factor 3

Yes: [55]

IPI00328715

216

LYRIC protein

Yes: [56]

IPI00410693

94

Splice Isoform 1 of Plasminogen activator inhibitor 1 RNA-binding protein

Yes: [57]

IPI00456731

123

Similar to Laminin receptor 1

Yes: UniProt P08865


(A) The table illustrates proteins identified in DRM fractions generated using the modified fractionation method that have previously been described as present in or associated with DRMs isolated by other methods. (B) The table lists proteins identified in the modified DRM (TI) fraction that are indicated to be nuclear-localized based on Gene Ontology Cellular Component terminology, but that have been reported to exist in other locations. Proteins that are restricted to nuclei are shaded in grey.

aLikely DRM-associated.

Adam et al. BMC Cell Biology 2008 9:30   doi:10.1186/1471-2121-9-30

Open Data