Table 1

Summary of sec26 mutants and genetic interactions with GLO3

Name

Mutations

Restrictive temperature1

GLO3 suppression?

glo3Δ synthetic lethality?


FW

F856A W860A

40°

YES

YES

TDD

T536A D903A D904A (sec26-1)

34°

YES

YES

7A4

K659R I681V D759V S810P C905S I938V L950S

37°

YES

YES

7D15

S725Y K732N N772I H774R F777Y F799I K915M K964T

37°

YES

YES

9C15

C678S F693S D759G Y823C V838A I842T Y913H

37°

YES

YES

10D22

R672G I727T V743G K807R T877A

37°

YES

YES

10D25

L651Q K659E H839P I846T F863I A959S

37–40°

YES

YES

11A3

K659E K679N I733L Y744C F754S Y832N A851V F863L I893T I938L V973A

37°

YES

YES

12B6

N680T R691W D741G F817Y M859V

37–40°

YES

YES

1A11

F856A W860A D904A C905S

37–40

YES

+/-

11D26

E633D I733T D741V T769P F802L (sec26-2)

37°

YES

+/-

12D18

Q661R S740P V806A F907Y F918S

37°

YES

+/-

11C15

S628P I785V M859I L950S D956V H972Y

37°

YES

+/-

7D20

S740P I785T N793S K801E F863S A951T

37°

YES

no

9A1

I598N S728T D759V D823G I846T H855L I869L G901R

37°

YES

no

10D24

V623A S628T P636A V757A T779S W860R K887R T895A K933E A962T

37°

YES

no

11B10

L773Q I795T H839L N966Y

37°

YES

no

11C13

Q607L M624V L646P H670P C678S I846T T877I N890S E920V K963E

34°

YES

no

9C16

S626P K655R N793H T803S G818S N836D K868R E929K Q948L K963R

37–40°

YES

no

5A7

E633G N635Y E663G K666R F690L R691W S728L V756D Q776R D921G S932T L950M

34°

no

YES

7C13

F650L G695S H797Q I821N W860L E902G

34°

no

YES

7D17

K655N A665T D676G Q702R Q776H G781C Y822N D844E F863Y R883S

37°

no

YES

7D18

K614R L780H V794E A825V L885M C910R C927S A962S

37°

no

YES

9A2

Q607R K669M I846F M859V F907L F918Y

40°

no

YES

9C14

D697N D704H S740T I820T R831C I842V I928F S932T

37°

no

YES

10C20

S608T D616E D676G F693L D704G S726G P735H E746K H774Y F799L K847I F856Y

34°

no

YES

10D26

I598T R622G A718P T722I P735L K767E H839P T850S N890S D921E N966Y T971A

37°

no

YES

11B11

I615F L646Q K715R F817S F863L K872E M891L L950S

34–37°

no

YES

11C14

R622G K679G A694D A924V C927S V937D

40°

no

YES

11D30

A649E C910R S917P

37°

no

YES

11C20

D676N L730R F754L N772Y A830D I834N D852V S917T F918I

37°

no

YES

7A5

S619F L620V D709E R957G

40°

no

+/-

11C19

V600D F658S L712Q Q753H Q776R N836I T965A

37°

no

+/-

7D16

S628T L712Q P789S V794E D829V I846F I961F2

34–37°

no

no

11B8

I615F R660G S724C S916P K964E L970H

40°

no

no

11B9

E617V I629S K715I T889S A924V A959H3

34°

no

no

11C16

I662F V701G H720P V743G K784R F856I K887E

40°

no

no

11C17

L620I M624I L646P T654S T686M I749V V794G N867S K868G

37°

no

no

11D25

S628P A718S I733M P796T A825V Y832F3

37–40°

no

no

12C13

S628P G798C F802S E866G Y913C

34°

no

no


1 restrictive temperature on SCGalactose media

2 also contains additional residues at COOH-terminus 974WTTLS978

3 also contains deletion of COOH-terminal residues 960–973

DeRegis et al. BMC Cell Biology 2008 9:3   doi:10.1186/1471-2121-9-3

Open Data