Table 1

Expression of mNek1 truncations disrupt centrosomal γ-tubulin.

mNek1 aa residues expressed with GFP tag

Subcellular localization of GFP-truncated Nek1

Percent of transfected cells without γ-tubulin foci


1–258

Cytoplasmic

0

1–352

Nuclear

0

1–407

Nuclear

8 ± 1

1–510

Nuclear

68 ± 3

1–599

Nuclear

64 ± 3

1–686

Nuclear

64 ± 4

1–686 (K33R)

Ciliary

0


Quantification of the loss of γ-tubulin foci is based on examination of 100 transfected (green) cells from each of three independent experiments. These were scored as either retaining or lacking detectable γ-tubulin foci. Shown is the average number of transfected cells that lack γ-tubulin foci, plus or minus the standard deviation. Constructs containing at least the first two coiled-coils significantly reduce the number of transfected cells retaining γ-tubulin foci (constructs 1–510 and 1–599).

White and Quarmby BMC Cell Biology 2008 9:29   doi:10.1186/1471-2121-9-29

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