The Coiled-coil domain of mNek1 contains a ciliary localization signal. (A-G) Representative images of eGFP-tagged mNek1 constructs containing the mutation K33R (green), γ- and acetylated-tubulin (red), and a merge with DAPI (blue). Localization to the primary cilium was scored by the co-localization of the acetylated-tubulin-stained cilium and the eGFP signal as either none (-), minimal (+), or strong (+++). Constructs that contain coiled-coil 2 localize to the primary cilium (D-G), and the coiled-coil domain is sufficient for localization to the primary cilium (G). Cells in these images were fixed and stained 12 hours after transfection. Bar, 10 μm.
White and Quarmby BMC Cell Biology 2008 9:29 doi:10.1186/1471-2121-9-29