Figure 2.

Transiently transfected constructs are first expressed as cells exit mitosis. (A) Representative fluorescence microscopy image of IMCD3 cells expressing eGFP (green) two hours post-transfection connected by a cytokinetic bridge stained with acetylated-tubulin (red). (B) Graph of the presence of cytokinetic structures and primary cilia over a post-transfection time course. Error bars represent the standard deviation of two experiments, n = 100 for each. The high percentage of transfected cells possessing cytokinetic structures two hours post-transfection indicates that these cells recently completed mitosis. Bar, 10 μm.

White and Quarmby BMC Cell Biology 2008 9:29   doi:10.1186/1471-2121-9-29
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