Additional file 2.

FRET images. FRET images were selected, which appear representative for low FRET-efficiency (<10%), medium FRET efficiency (~20%) and high FRET efficiency (>40%). CFP and YFP denominate the reference channels. "raw FRET" displays the recorded emission in the FRET channel without correction for CFP-crosstalk and YFP-direct excitation. CFP-crosstalk and YFP-direct excitation are considered in "corr. FRET".

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Seidel et al. BMC Cell Biology 2008 9:28   doi:10.1186/1471-2121-9-28