Subcellular localisation of VO-subunits and proteolipid constructs. VHA-c" (A, D), truncated VHA-c" (B), the VHA-c/VHA-c" hybrid proteolipid subunit (C) as well as VHA-e isoforms (E, F) were genetically fused to YFP and transiently expressed in Arabidopsis mesophyll protoplasts. The YFP fluorescence as shown in green was excited with 514 nm excitation and detected in the range of 530–600 nm by confocal microscopy. Chlorophyll autofluorescence is displayed in red and was detected in the range of 650–700 nm with 458 nm excitation. A, D: VHA-c"1. B: VHA-c"trunc. C: hybrid proteolipid. E: VHA-e2. F: VHA-e1. Scale bars represent 20 μm.
Seidel et al. BMC Cell Biology 2008 9:28 doi:10.1186/1471-2121-9-28