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Open Access Research article

Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis

Thorsten Seidel1*, Daniel Schnitzer1, Dortje Golldack1, Markus Sauer2 and Karl-Josef Dietz1*

Author Affiliations

1 Department of Biochemistry and Physiology of Plants, W5, University of Bielefeld, 33501 Bielefeld, Germany

2 Department of Laser Physics, D3, University of Bielefeld, 33501 Bielefeld, Germany

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BMC Cell Biology 2008, 9:28  doi:10.1186/1471-2121-9-28

Published: 28 May 2008

Additional files

Additional file 1:

FRET-measurements. The following FRET-efficiencies were measured and displayed in a model of the subsector V0: A) FRET-efficiencies between VHA-e isoforms and VHA-a, VHA-c and VHA-c", respectively. B) FRET-efficiencies between VHA-a and proteolipid subunits as well as between VHA-c and proteolipid subunits. C) Substitution of two VHA-c by two truncated VHA-c results in a reduced proteolipid ring diameter and hence in increased FRET-efficiency.

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Additional file 2:

FRET images. FRET images were selected, which appear representative for low FRET-efficiency (<10%), medium FRET efficiency (~20%) and high FRET efficiency (>40%). CFP and YFP denominate the reference channels. "raw FRET" displays the recorded emission in the FRET channel without correction for CFP-crosstalk and YFP-direct excitation. CFP-crosstalk and YFP-direct excitation are considered in "corr. FRET".

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Additional file 3:

FRET efficiencies. The table contains the observed FRET-efficiencies. The mean average ± SE is shown.

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Additional file 4:

List of constructs.

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