Table 1

Primers used in the study

S. No.

Clone

Primer No.

Sequence

Restriction sites


1

NP-FL

35104

5'-cgc

    gaattc
atggcgaccaaaggc

EcoRI

35575

3'-gacccc

    gggccc
attgtcgtactcctc

ApaI

2

obpNLS+NC

36823

5'-cgc

    gaattc
atccaaatgtgcaccg

EcoRI

62638

3'-cgc

    gggccc
tttgatcattctgat

ApaI

3

obpNLS+NTer

39815

5'-ggg

    ctcgag
catccaaatgtgc

XhoI

39821

3'-gcg

    gaattc
gcgccagattcgtct

EcoRI

4

obpNLS+CTer

39820

5'-ggg

    ctcgag
caagaaaactggagga

XhoI

62638

3'-cgc

    gggccc
tttgatcattctgat

ApaI

5

bpNLS-1R

39815

5'-ggg

    ctcgag
catccaaatgtgc

XhoI

39813

3'-gcg

    cccggg
tcatctcctccactt

ApaI

6

bpNLS-1M

39815

5'-ggg

    ctcgag
catccaaatgtgc

XhoI

39818

3'-gcg

    cccggg
tcatctcatccactt

ApaI

7

bpNLS-2R

39814

5'-gcg

    gaattc
aggagagaactcatc

EcoRI

62638

3'-cgc

    gggccc
tttgatcattctgat

ApaI

8

bpNLS-2M

39819

5'-gcg

    gaattc
atgagagaactcatc

EcoRI

62638

3'-cgc

    gggccc
tttgatcattctgat

ApaI

9

NPΔ obpNLS

39815

5'-ggg

    ctcgag
catccaaatgtgc

XhoI

40466

3'-gcg

    gaattc
aggatctttccc

EcoRI

39817

5'-gag

    gaattc
caagctaataatggt

EcoRI

62638

3'-cgcgggccctttgatcattctgat

ApaI

10

NP41–89

39815

5'-ggg

    ctcgag
catccaaatgtgc

XhoI

40466

3'-gcg

    gaattc
aggatctttccc

EcoRI

11

NP122–197

39817

5'-gag

    gaattc
caagctaataatggt

EcoRI

62638

3'-cgcgggccctttgatcattctgat

ApaI

12

2bpNLS+NSP6

39820

5'-ggg

    ctcgag
caagaaaactggagga

XhoI

39821

3'-gcg

    gaattc
gcgccagattcgtct

EcoRI


Ketha and Atreya BMC Cell Biology 2008 9:22   doi:10.1186/1471-2121-9-22

Open Data