Schematic representation of NP-full-length and deletion constructs. Various NP-FL and deletion clones were made between the nNLS and cNLS regions. GFP is represented by chequered-box; NLS regions are indicated by darkly-shaded box; nuclear accumulation signal (NAS) is indicated by striped-box; rotavirus NSP6 is represented by an empty, unshaded box. Critical amino acid (R or M) at position 105 is indicated by larger font size and an asterisk. Cellular localization of each construct is indicated by N (nuclear), N>C (nuclear greater than cytoplasm), C>N (cytoplasm greater than nuclear), and C (cytoplasmic).
Ketha and Atreya BMC Cell Biology 2008 9:22 doi:10.1186/1471-2121-9-22