Spindle Morphologies in kif4-/380 K cells. Representative examples of normal and aberrant mitotic spindles in kif4-/380 K cultures. The two left-most panels show cells with normal appearing metaphase arrangements, with bipolar spindle MTs (in green) that flank condensed chromosomes (blue). These two figures are indistinguishable from mitotic wild type cells. The remaining four panels show examples of aberrant mitotic figures (multipolar spindles, supernumerary or fragmented spindle poles, asynchronous chromatin condensation) that are not normally found in wild-type, kif4-, or 380 K cells alone. This panel suggests that a combination of dynein and Kif4 (kinesin-7, CENP-E) activity is required for proper spindle assembly. Scale bar = 5 μm.
Nag et al. BMC Cell Biology 2008 9:21 doi:10.1186/1471-2121-9-21