Isolation, culture and differentiation of ChDPSCs. (a) Spindle and fibroblast-like cells, similar to those of the hBMSCs, were observed in ChDPSC culture. (b) Osteogenic and (c) Adipogenic differentiation were demonstrated by three weeks induction followed by Alizarin Red S and Oil-Red O staining, respectively. (d) Chondrogenic differentiation was demonstrated by four weeks induction followed by Alcian blue staining of cryosection. Bar = 5 μm.
Cheng et al. BMC Cell Biology 2008 9:20 doi:10.1186/1471-2121-9-20