Additional file 2.
Real-time translocation of proteins using HaloTag Technology. Timelapse imaging of live U2OS cells sequentially labeled with HaloTag 488 and TMR ligands shows real-time translocation of both protein pools. After labeling, time 0 shows spatial separation of the green surface protein around the red internal protein. Imaging every 20 minutes over 17 hours first shows the green surface pool internalizing and the red internal pool trafficking to the surface, then the original green surface pool inside and the red pool on the membrane, and finally the red surface pool internalizing.
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Svendsen et al. BMC Cell Biology 2008 9:17 doi:10.1186/1471-2121-9-17