Table 1

List of antibodies, suppliers and concentrations used

Antigen

Isotype

Suppliers

Concentration


E-cadherin

mouse IgG2A

BD Biosciences, #610181 (Oxford, UK)

0.25 μg/ml

Ksp-cadherin

mouse IgG1

Zymed, clone 4H6/F9, #18–7383 (InVitrogen)

0.4 μg/ml

collagen IV

rabbit polyclonal

Acris, #R1041 (Herford, Germany)

5.0 μg/ml

Ck 8/18

mouse IgG1

Novocastra, clone 5D3, #5D3CE (Newcastle upon Tyne, UK)

0.94 μg/ml

vimentin

mouse IgG2A

Dako, clone Vim 3B4, #M7020

0.25 μg/ml

ER

rabbit polyclonal

Labvision, clone SP1, #RM-9101-S1 (Runcorn, Cheshire, UK)

1:400

αSMA

mouse IgG2A

Sigma, #A2547 clone 1A4

0.88 μg/ml


Second layer antibody

Company

Concentration


Alexa488-conjugated goat anti-mouse IgG2A

Invitrogen, #A21131

2.0 μg/ml

Alexa488-conjugated goat anti-mouse IgG1

Invitrogen, #A21121

2.0 μg/ml

Alexa555-conjugated goat anti-mouse IgG1

Invitrogen, #A21127

2.0 μg/ml

Alexa555-conjugated goat anti-rabbit Ig

Invitrogen, #A21429

2.0 μg/ml

Alexa633-conjugated goat anti-mouse IgG2A

Invitrogen, #A21136

2.0 μg/ml


Robertson et al. BMC Cell Biology 2008 9:13   doi:10.1186/1471-2121-9-13

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