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List of antibodies, suppliers and concentrations used |
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| Antigen |
Isotype |
Suppliers |
Concentration |
|
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| E-cadherin |
mouse IgG2A |
BD Biosciences, #610181 (Oxford, UK) |
0.25 μg/ml |
| Ksp-cadherin |
mouse IgG1 |
Zymed, clone 4H6/F9, #18–7383 (InVitrogen) |
0.4 μg/ml |
| collagen IV |
rabbit polyclonal |
Acris, #R1041 (Herford, Germany) |
5.0 μg/ml |
| Ck 8/18 |
mouse IgG1 |
Novocastra, clone 5D3, #5D3CE (Newcastle upon Tyne, UK) |
0.94 μg/ml |
| vimentin |
mouse IgG2A |
Dako, clone Vim 3B4, #M7020 |
0.25 μg/ml |
| ER |
rabbit polyclonal |
Labvision, clone SP1, #RM-9101-S1 (Runcorn, Cheshire, UK) |
1:400 |
| αSMA |
mouse IgG2A |
Sigma, #A2547 clone 1A4 |
0.88 μg/ml |
|
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| Second layer antibody |
Company |
Concentration |
|
|
|
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| Alexa488-conjugated goat anti-mouse IgG2A |
Invitrogen, #A21131 |
2.0 μg/ml |
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| Alexa488-conjugated goat anti-mouse IgG1 |
Invitrogen, #A21121 |
2.0 μg/ml |
|
| Alexa555-conjugated goat anti-mouse IgG1 |
Invitrogen, #A21127 |
2.0 μg/ml |
|
| Alexa555-conjugated goat anti-rabbit Ig |
Invitrogen, #A21429 |
2.0 μg/ml |
|
| Alexa633-conjugated goat anti-mouse IgG2A |
Invitrogen, #A21136 |
2.0 μg/ml |
|
Robertson et al. BMC Cell Biology 2008 9:13 doi:10.1186/1471-2121-9-13 |
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