Figure 2.

Expression of cytokeratin 8/18, oestrogen receptor and vimentin in normal human breast and breast cancer. 3 μm FFPE sections of human adult breast containing both normal tissue and columnar cell change, and an ER-positive breast cancer showing areas of DCIS and invasive ductal carcinoma were stained anti-Ck 8/18 mAb, anti-vimentin mAb and anti-ER Ab followed by Alexa488 goat anti-mouse IgG₁, Alexa633 goat anti-mouse IgG_2A and Alexa555 goat anti-rabbit Ig. Nuclei were counterstained with DAPI. In all images colours are as follows: Ck 8/18 (green), ER (red), vimentin (yellow), DAPI (blue). A. 4 colour images, arrowheads indicate Ck 8/18-negative myoepithelial cells. B. Ck 8/18 and ER, arrows indicate ER expression in luminal epithelial cells with high Ck 8/18 expression in the normal breast. C. Ck 8/18 and vimentin, arrows indicate vimentin expression in the myoepithelial cells of columnar cell change, arrowheads indicate vimentin-positive, Ck 8/18-negative cells within the DCIS. D. Ck 8/18, ER and vimentin. Scale bar, 50 μm.