Expression of E-cadherin, Ksp-cadherin and collagen IV in kidney. 3 μm FFPE sections of adult human kidney were dewaxed, rehydrated and subject to antigen retrieval. A. For immunoperoxidase labelling, sections were incubated with the indicated antibodies and detection was achieved with the Vectastain avidin-biotin complex (ABC) system used according to the manufacturer's protocol (Vector Laboratories). Slides were counterstained with haematoxylin. B. Sections were stained as described in the Methods with anti-E-cadherin mAb and anti-Ksp-cadherin mAb or with anti-E-cadherin mAb and anti-collagen IV Ab followed by Alexa488-conjugated goat anti-mouse IgG2A and Alexa555 goat anti-mouse IgG1 or Alexa488-conjugated goat anti-mouse IgG2A and Alexa555 goat anti-rabbit Ig. Nuclei were counterstained with DAPI. Arrows indicate tubules expressing both cadherins, arrowheads indicate tubules expressing neither cadherin, * indicates tubules expressing E-cadherin alone. Scale bar, 50 μm.
Robertson et al. BMC Cell Biology 2008 9:13 doi:10.1186/1471-2121-9-13