Additional File 1.
Activation of β-catenin pathway by BIO. In order to evaluate the activation of the β-catenin pathway, hMADS cells were nucleofected as described in Zaragosi et al.  with a plasmid carrying firefly luciferase under the control of a Tcf/Lef response element (TopLuc). An inactive version of the response element was used as a control (FopLuc). Twenty four hours after nucleofection, cells were treated with BIO and 24 h after BIO treatment, cells were analyzed for luciferase expression. Renilla luciferase was co-nucleofected and used for normalization. Data indicated that β-catenin pathway was activated in hMADS cells after inhibiting GSK3.
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Zaragosi et al. BMC Cell Biology 2008 9:11 doi:10.1186/1471-2121-9-11