Effects of BIO treatment during the first 3 days of differentiation on adipogenic and osteogenic abilities of hMADS cells. hMADS3 cells were induced to undergo differentiation into the indicated lineages in the presence of BIO and MeBIO during the first 3 days. Then, compounds were withdrawn and GPDH and ALP enzymatic activities were quantified at day 10. *: p < 0.05, **: p < 0.01, NS: not significant (A). hMADS3 cells were induced to undergo differentiation into adipocytes (upper panel) or osteoblasts (lower panel) as in A) and RNAs were prepared at day ten. The expression of molecular markers was checked by qPCR (B). Results are the log2 inductions of BIO treated cells versus MeBIO treated cells. Expression data are colour-coded according to the scale that is displayed.
Zaragosi et al. BMC Cell Biology 2008 9:11 doi:10.1186/1471-2121-9-11