Morphological examination of the testis tissues from young wild-type (panels A and C) and Mer3 (panels B and D) males. The control FM6 and Mer3 mutant males were obtained according to Materials and Methods. The DAPI-stained testis from the control male contained a bulging seminal vesicle (SV) (A). In contrast, a shriveled seminal vesicle was seen in the Mer3 testis (B). T, testis; AG, accessory gland. Acetic acid/orcein staining revealed that the testis tissue from the control male contained well-organized sperm bundles (arrows) (C), while the Mer3 testis had fewer sperm in a more disorganized bundle (arrows) (D).
Dorogova et al. BMC Cell Biology 2008 9:1 doi:10.1186/1471-2121-9-1