Colocalization of CLIC1 with markers of plasma membrane and nucleus following digitonin extraction. Panc1 cells were grown on glass coverslips and extracted with digitonin prior to PLP fixation and staining for CLIC1 (red) and subcellular compartment markers (green). The right column in each set is a merged image generated from the red (left column) and green (center column) channels. A, B. Two separate fields stained for CLIC1 (red) and Integrin α2 (green), a plasma membrane marker. C. Cells stained for CLIC1 (red) and Annexin2 (green), a plasma membrane marker. D. Cells stained for CLIC1 (red) and Nucleoporin p62 (green) a marker of nuclear envelope. The scale bar in panel A represents 20 μm and applies to all panels.
Ulmasov et al. BMC Cell Biology 2007 8:8 doi:10.1186/1471-2121-8-8