Figure 1.

Characterization of antibodies. A. Lysates of bacteria expressing glutathione-S-transferase (GST, lane 1) or GST-CLIC1 (lane 2), GST-CLIC4 (lane 3) or GST-CLIC5 (lane 4) fusion proteins were separated by SDS-PAGE and stained with Coomassie blue. Lane M contains molecular mass standards of 96, 66, 45, 31, and 21 kDa. B. Gel identical to that shown in panel A, blotted and probed with AP1089. C. Gel as in B, probed with 9F5. D. Mouse kidney microsomal membranes (lane 1) or whole cell lysates from Panc1 cells (lane 2), HELA cells (lane 3) or HELA cells overexpressing exogenous CLIC1 (lane 4) were separated on a 10% SDS-polyacrylamide gel, blotted, and probed with AP1089. Sample lanes each contained 30 μg of protein. Migration positions of molecular mass standards of 96, 66, 45, and 31 kDa are indicated. E. Whole cell lysates from Panc1 cells (lane 1), T84 cells (lane 2) or mouse kidney microsomal membranes (lane 3) were separated on 12% SDS-polyacrylamide gels, blotted, and probed with 9F5 monoclonal antibody. Lanes contain 5 μg of each protein sample. Migration positions of molecular mass standards of 66, 45, 31, and 21 kDa are indicated.

Ulmasov et al. BMC Cell Biology 2007 8:8   doi:10.1186/1471-2121-8-8
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