Table 1

Influence of transduction with recombinant adenovirus (AdGFP) or baculovirus (BacGFP) on phosphorylation of Akt Ser-473 in SHSY-5Y and C3A cells.

Cells

Virus

MOI

Akt (A.U.)

pSer473 Akt (A.U.)

pSer473 Akt/Akt


10 min

30 min

10 min

30 min

10 min

30 min


SHSY-5Y

AdGFP

0

2.73

2.46

0.20

0.32

0.07

0.13

SHSY-5Y

AdGFP

10

2.60

2.24

0.81

0.90

0.31

0.40

SHSY-5Y

AdGFP

50

2.25

2.03

0.80

1.30

0.35

0.64

C3A

AdGFP

0

0.80

0.95

0.15

0.24

0.19

0.25

C3A

AdGFP

10

0.72

0.64

0.25

0.45

0.35

0.70

C3A

AdGFP

50

0.69

0.59

0.31

0.54

0.45

0.91


SHSY-5Y

BacGFP

0

2.64

2.56

0.25

0.36

0.09

0.14

SHSY-5Y

BacGFP

1000

2.74

2.62

0.20

0.40

0.07

0.15

SHSY-5Y

BacGFP

10000

2.86

2.69

0.22

0.36

0.08

0.13

C3A

BacGFP

0

0.94

0.79

0.16

0.20

0.17

0.25

C3A

BacGFP

1000

1.04

0.84

0.15

0.20

0.15

0.23

C3A

BacGFP

10000

1.09

0.89

0.15

0.23

0.14

0.26


Time notations refer to time of incubation at experimental conditions determined by the ELISA method.

Andersson et al. BMC Cell Biology 2007 8:6   doi:10.1186/1471-2121-8-6

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