Inhibitors of PKC do not significantly inhibit carbachol-induced dispersion. Isolated RPE cells were first treated with 10 μM forskolin for 45' to induce aggregation. Following aggregation, cells were treated with 100 nM carbachol (Carb) along with either 100 nM or 500 nM staurosporine (Staur), a PKC-inhibitor. The mean pigment indices of cells treated with staurosporine at these concentrations were not statistically significantly different than the mean pigment index of cells treated with carbachol alone. Bisindolylmaleimide II (BisII), a second PKC-inhibitor, was also applied to cells in the presence of carbachol following aggregation. The pigment index of cells treated with 20 nM or 2 μM bisindolylmaleimide II was not significantly different from the pigment index of cells treated with carbachol alone. However, the pigment index of cells treated with 200 nM bisindolylmaleimide was statistically significantly different, and dispersion was inhibited by 20%.
Johnson and García BMC Cell Biology 2007 8:53 doi:10.1186/1471-2121-8-53