Effect of phosphatase inhibitors on Borealin. The mobility shift of Borealin was determined by Western blotting using 12.6% SDS-polyacrylamide gel. Transfers were stripped and reprobed for β-actin as a loading control. (A) Effect of NaF on the migration of exogenous and endogenous Borealin. WT-8 cells blocked in S phase using hydroxyurea were either left untreated or treated with the indicated doses of NaF for 3.5 hours. (B) Effect of okadaic acid and cyclosporine A on Borealin. Asynchronously growing WT-8 cells were treated with either okadaic acid or cyclosporine A or both for 2 hours. As a control for the shift, cells were treated with CHX for 2 hours.
Kaur et al. BMC Cell Biology 2007 8:5 doi:10.1186/1471-2121-8-5