Effect of Apg-2 and ZO-1 depletion on the organisation of 3-D cultures. Control, and Apg-2 and ZO-1 RNAi cell lines were cultured as in figure 6. After fixation, the samples were labelled with antibodies against β-catenin and podocalyxin/GP135 (A); occludin, erbB-2 and with Alexa654-phalloidin (B); or with TRITC-phalloidin and anti-ZO-1 antibodies (C). Note, erbB-2 does not only localise to the lateral plasma membrane but also to cytoplasmic organelles, causing the cytoplasmic staining between the nucleus in the apical membrane in control cultures (B). Residual ZO-1 staining in ZO-1 RNAi cells was detected at cell-cell contacts (C).
Aijaz et al. BMC Cell Biology 2007 8:49 doi:10.1186/1471-2121-8-49