Quantification of 3-D morphogenesis assays. Control and Apg-2 RNAi cell lines were cultured and processed as in figure 6. In two experiments performed with different batches of matrigel, pictures from ten different areas were taken and quantified by counting the number and types of cultures. The formed structures were divided into the following classes: spherical structures with a single lumen; 2 lumen and 3 (or more lumen) but with a normal polarised organisation; and all other structures including cysts with no lumen.
Aijaz et al. BMC Cell Biology 2007 8:49 doi:10.1186/1471-2121-8-49