Figure 7.

Apg-2 depletion inhibits epithelial morphogenesis in 3-D cultures. Control and Apg-2 RNAi cell lines were cultured as in figure 6. After fixation, the samples were labelled with FITC-phalloidin and antibodies against β-catenin (shown in red), the Golgi marker GP73 (shown in blue), and for DNA (shown in white). Panel B shows confocal sections taken at a higher magnification than those in panel A. Note, cysts formed by Apg-2 depleted cells were often not normally polarised and had irregular shapes.