Quantitative phototaxis of strains lacking repeat segments. Phototaxis at defined cell densities was performed on wild type AX2, the filamin null mutant HG1264, the rescued strain HPF620 and the strains expressing filamin lacking one of the repeat segments as indicated by the cartoon under the strain name. The trails were digitised and analysed using directional statistics to determine their accuracies of phototaxis (κ) at the defined cell densities. Vertical bars represent 90% phototaxis than the wild type AX2, the original mutant (HG1264) displayed poor accuracies, as did all of the strains lacking repeat segments 2–6. Two transformants expressing filamin with a deletion of repeat one showed accuracies of phototaxis of approximately 1% (HPF622) or 50% (HPF623) of the wild type values.
Annesley et al. BMC Cell Biology 2007 8:48 doi:10.1186/1471-2121-8-48