Complementation of filamin null mutant HG1264 depends upon the level of filamin expression. (A) Western blot of proteins from AX2 and two filamin mutant transformants, HPF629 and HPF639 ectopically expressing filamin. The two transformants expressed filamin at a lower level than that observed in the wild type. (B) The slug trails of HPF629 were digitised and this transformant displayed a defective phototaxis phenotype as observed in the filamin mutant from which this strain was derived, HG1264. The light source is to the right of the figure. (C) Western blot using the antifilamin antibody against total cellular protein from AX2 (lane 1, wild type), HG1264 (lane 2, filamin null mutant) and HPF620 (lane 3, filamin mutant ectopically expressing filamin). No filamin can be detected in the filamin mutant whereas filamin is expressed in HPF620 to a level comparable to that of the wild type. (D) The slug trails of HPF620 were digitised and this transformant displayed a wild type phototaxis phenotype. The light source is to the right of the figure.
Annesley et al. BMC Cell Biology 2007 8:48 doi:10.1186/1471-2121-8-48