Table 3

Plasmids used in this study

Plasmid

Marker

Source


pIH4.1

URA3 CEN FUS3-GFP

[52]

pIH4.1AA

URA3 CEN FUS3T180AY182A-GFP

[52]

YCplac22/FUS3-GFP (AOB117)

TRP1 CEN FUS3-GFP

[52]

YCplac22/FUS3T180AY182A-GFP (TBB182)

TRP1 CEN FUS3T180AY182A-GFP

[52]

pRS316CG/MSG5-GFP (HKB101)

URA3 CEN MSG5-GFP

[52]

YCplac111/GAL1-MSG5 (HKB100)

LEU2 CEN GAL1-MSG5

[52]

YCplac22/GAL1-MSG5 (AEB101)

TRP1 CEN GAL1-MSG5

[52]

YCplac111/GAL1-MSG5C319A (TBB109)

LEU2 CEN GAL1-MSG5C319A

This study

YCplac22/GAL1-STE12 (TBB157)

TRP1 CEN GAL1-STE12

This study

YCplac22/NLS-MSG5M45A (TBB172)

TRP1 CEN NLS-MSG5M45A

This study

YCplac22/NES-MSG5M45A (TBB174)

TRP1 CEN NES-MSG5M45A

This study

YCplac33/NLS-MSG5M45A (TBB181)

URA3 CEN NLS-MSG5M45A

This study

YCplac33/NES-MSG5M45A (TBB180)

URA3 CEN NES-MSG5M45A

This study

YCplac22/NLS-MSG5M45AC319A (TBB178)

TRP1 CEN NLS-MSG5M45AC319A

This study

YCplac22/NES-MSG5M45AC319A (TBB176)

TRP1 CEN NES-MSG5M45AC319A

This study

pRS316CG/NLS-MSG5-GFPM45A (TBB171)

URA3 CEN NLS-MSG5M45A-GFP

This study

pRS316CG/NES-MSG5-GFPM45A (TBB170)

URA3 CEN NES-MSG5M45A-GFP

This study

YEplac181/PTP2-GFP

LEU2 2 μ PTP2-GFP

[84]

YEplac181/PTP3-GFP

LEU2 2 μ PTP3-GFP

[84]


Blackwell et al. BMC Cell Biology 2007 8:44   doi:10.1186/1471-2121-8-44

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