Additional file 1.

RNCF calculations for strains listed in Table 1. Description: Strains of the indicated genotype were transformed with Fus3-GFP (A and E-I) or Fus3T180AY182A-GFP (B-D) and grown to mid-log phase. The cultures were then split and grown with or without the addition of 12 nM pheromone. Images were acquired from the treated and untreated cells three hours later. The RNCF values were determined as described in the Materials and Methods, and their distributions represented in histograms. The number of cells (y axis) is plotted as a function of the RNCF values (x axis). Mean RNCF values are indicated ± s.d. In each panel, the untreated and treated cultures are represented by the left and right histograms, respectively. Arrows indicate the mean RNCF value for each culture. (A)Wild type cells with Fus3-GFP; (B) Wild type cells with Fus3T180AY182A-GFP; (C) dig1Δ cells; (D)dig2Δ cells; (E) Msg5 overexpression cells; (F) MSG5M45AC319A overexpression cells; (G) NES-MSG5M45A/NLS-MSG5M45A cells; (H) NES-MSG5M45A/NLS-MSG5M45AC319A cells; (I) NES-MSG5M45AC319A/NLS-MSG5M45A cells.

Format: PNG Size: 344KB Download file

Blackwell et al. BMC Cell Biology 2007 8:44   doi:10.1186/1471-2121-8-44