Ste12 overexpression causes hyper-accumulation of Fus3 in the nuclei of pheromone-treated cells. Wild type cells were transformed with either the Fus3-GFP reporter or the Fus3T180AY182A-GFP reporter, and either the GAL1-STE12 plasmid or an empty vector. Strains were grown to mid-log phase in sucrose medium and galactose was added to a concentration of 2% two hours before pheromone treatment (GAL1 promoter on). The cultures were then split and grown with or without the addition of 12 nM pheromone. Images were acquired from the untreated and treated cultures in 2 hour intervals. The mean RNCF ± s.d. values were determined as described in the Materials and Methods, and are represented in histograms as follows: wild type cells expressing Fus3-GFP–untreated (white bars) and treated (dark gray bars); wild type cells expressing Fus3-GFP and overexpressing Ste12–untreated (light gray bars) and treated (black bars); wild type cells expressing Fus3T180AY182A-GFP and overexpressing Ste12–untreated (stippled bars) and treated (hatched bars). Asterisks indicate statistically significant differences between strains with ρ = 0.003. See additional file 2 for images representative of each time point sampled for Figure 2.
Blackwell et al. BMC Cell Biology 2007 8:44 doi:10.1186/1471-2121-8-44