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Open Access Highly Accessed Methodology article

Identifying genes preferentially expressed in undifferentiated embryonic stem cells

Xiajun Li and Philip Leder

Author Affiliations

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA

BMC Cell Biology 2007, 8:37  doi:10.1186/1471-2121-8-37

Published: 28 August 2007

Additional files

Additional file 1:

Supplemental figures. Northern blot as shown in Supplemental Figure S1 was used to examine the levels of GFP hybrid transcripts of some candidate ES clones that display significant reduction of GFP fluorescence in FACS profiling. Supplemental Figure S2 shows the GFP FACS profiles of both undifferentiated cells and differentiated cells derived from the candidate ES clone 5C32. Supplemental Figure S3 provides evidence suggesting that an EGFP/Neo fusion protein is toxic to the cells.

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