Figure 5.

Carbohydrate compositions by Mass spectrometry. A. Structural characterization by negative electrospray ionization mass spectrometry. MS analysis has been carried out on oligosaccharides obtained from partial hydrolysis of bovine liver filaments. Spectrum was acquired on Q-Tof micro (Waters) with direct injection through capLC to nanospray ESI in negative ion mode. B. Mass spectrum of CACO2 straw cells. Partial hydrolysis with 1N HCl produced linear acidic glucose polymers that differed by neutral loss of sulphated glucose (242.0). Spectra from polysaccharides of those found in urine, such as hyaluronate and dermatan, were acquired as references.

Wu et al. BMC Cell Biology 2007 8:36   doi:10.1186/1471-2121-8-36
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