Table 1

List of proteins purified from hydra lysates by 14-3-3-affinity chromatography

identification

protein score (matched peptides)

size of hydra protein (kDa)

size of gel band (kDa)

14-3-3 binding motif

previously identified 14-3-3 target


    cytoskeleton

tubulin α

102 (6)

53

53

no

[32]

tubulin β

89 (5)

50

50

yes

[32]

actin

186 (8)

41

40

yes

[27, 32]

tropomyosin

101 (7)

27

37

yes

[32]

    calcium signalling

calmodulin

71 (6)

17

17

no

[30, 32]

calcium binding prot.

134 (9)

20

20

no

calcium adaptor AIF-1

112 (7)

17

17

no

    metabolism

fru-1,6-BP-aldolase

98 (6)

36

41

no

GAPDH

172 (9)

36.5

37

yes (3)

[26, 28, 32]

PEPCK

53 (3)

71 (hs)

64

yes (hs)

ATP-synthase β

217 (13)

56

50

yes (2)

[25, 32]

    protein biosynthesis/-folding

EF-2

51 (3)

97

96

yes

[32]

EF1 α

156 (8)

51

50

yes (2)

[32]

eIF-3 β

132 (5)

36

36

yes (2)

Hsp70

174 (10)

75

70

yes

[29, 31, 32]

PDI-like protein

78 (6)

48 (dm)

50

yes

    Miscellaneous

ppod3

212 (10)

30

30

no

ppod4

290 (8)

32

32

yes

14-3-3 HyA

157 (6)

28.6

30

-

14-3-3 HyB

187 (8)

28.1

30

-

unknown protein

51 (4)

19

32

no

hypothetical protein

95 (6)

24

80

no


Protein scores and numbers of identified peptides from analysis of mass spectra using Mascot™ Software are given in column 2, columns 3 and 4 compare the molecular weights of the identified proteins with the apparent molecular weights of these proteins on the SDS-PAGE gel from which they had been cut out for analysis. In column 5 the presence of a conserved 14-3-3 binding motif in the sequence of the identified proteins is indicated [2, 3]. Column 6 lists citations that have described interactions of 14-3-3 proteins with plant or animal homologues of the identified proteins before. Abbreviations: dm: Drosophila melanogaster; hs: Homo sapiens

Pauly et al. BMC Cell Biology 2007 8:31   doi:10.1186/1471-2121-8-31

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