Figure 3.

Fractions after elution from 14-3-3 affinity column. (A) SDS PAGE gel of fractions eluted from Bmh1/2-affinity column, Western blot probed with DIG labelled Bmh1/2 from yeast. 14-3-3 binding proteins are only in the fraction that was eluted with 14-3-3 consensus peptide C (lane 5). (B) SDS-PAGE gel stained with coomassie; lanes 1 and 3 show size markers as indicated.