Additional File 6.
siRNA Western Blot. (A) Lysates were prepared as described in Methods and 100 μg were loaded onto a 10% SDS-PAGE gel, transferred to a PVDF membrane, and immunoblotted with specific EHD anti-sera as shown. Relative molecular weight (MW) markers are indicated in kiloDaltons (kD). (B) The percentage (%) of remaining EHD proteins after siRNA treatment was calculated by normalizing the intensity of the EHD band with respect to the loading control and comparing it with the bands in the control siRNA-treated lanes.
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George et al. BMC Cell Biology 2007 8:3 doi:10.1186/1471-2121-8-3