Figure 4.

Arf6 increases Kalirin5-induced Rac1 activation. COS7 cells were co-transfected with AU5-tagged Rac1 and with FLAG-tagged SR5-9 or FLAG-tagged Kalirin5 and with Arf6 or Arf6T27N. For each sample, total cell lysates were incubated with immobilized GST-Pak-CRIB and bound AU5-Rac (AU5-Rac-GTP) was detected by immunoblotting. Total cell lysates were also analyzed by immunoblotting. Graph depicts amount of AU5-Rac1-GTP relative to that observed in Kalirin 5 expressing cells. Mean ± s.d. of three independent experiments; **p < 0.01 versus Kalirin5 transfectants (one way ANOVA).