Arf6 recruits Kalirin5 to the PM and endosomes and enhances ruffling. A, B, D, E. Expression of Kalirin5 (K5), SR5 or Arf6 alone (A) or co-expressed with wild type Arf6 (B), Arf6T27N (D) or EFA6 (E). HeLa cells were transfected with FLAG-tagged Kalirin5 or SR5 alone, or with wild type Arf6, Arf6T27N or EFA6. After 18 h, cells were washed, fixed, and processed for indirect immunofluorescence to detect the localization of the overexpressed proteins. C. Increased association of Kalirin with membrane fraction in Cos7 cells expressing Arf6. Rapid fractionation of membrane and cytosol was performed on cells expressing FLAG-Kalirin5 alone or co-expressed with Arf6. The percentage of total Kalirin that was recovered in the membrane pellet is shown for three independent experiments (mean ± s.d.). **p < 0.01 versus Arf6 and Kalirin5 co-transfectants (one way ANOVA).
Koo et al. BMC Cell Biology 2007 8:29 doi:10.1186/1471-2121-8-29