Smad7 co-localizes with early endosomal marker, EEA1, on cytoplasmic vesicles, but not to an appreciable degree with Caveolin-1, a marker for caveolae, in E7 chicken lenses. A. Smad7 (red), EEA1 (green) and TOPRO (blue). B. Smad7 (red), Caveolin-1 (green) and TOPRO (blue). The insets show 2X magnified images of antibody stained cytoplasmic vesicles, more clearly demonstrating co-localization (yellow) for Smad7 and EEA1, but much less so for Smad7 and Caveolin-1.
Rajagopal et al. BMC Cell Biology 2007 8:25 doi:10.1186/1471-2121-8-25