Table 1

EC50 values of PAR agonists in A431. EC50 values were obtained using conventional Ca2+ flux assay, in comparison with those obtained using the MRCAT. In the case of MRCAT data, the amplitudes of both P-DMR and N-DMR events as a function of agonist concentration (as indicated in Fig. 1c) were used to calculate EC50.

Ligand

EC50 (n = 3)


Ca2+ flux assay

P-DMR

N-DMR


Trypsin

45.7 ± 5.8 nM

98.0 ± 27.8 nM

102.1 ± 21.9 nM

SLIGRL-amide

2.5 ± 0.3 μM

2.3 ± 0.6 μM

3.2 ± 0.8 μM

SLIGKV-amide

3.8 ± 0.4 μM

6.1 ± 1.0 μM

9.1 ± 2.2 μM

Thrombin

6.0 ± 1.0 unit/ml

9.6 ± 2.0 unit/ml

11.0 ± 1.9 unit/ml

SFLLR-amide

5.0 ± 0.4 μM

1.9 ± 0.1 μM

3.1 ± 0.2 μM


Fang and Ferrie BMC Cell Biology 2007 8:24   doi:10.1186/1471-2121-8-24

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