Cholesterol removal attenuates the agonist-induced DMR signals. mβCD was used to deplete cell surface cholesterol. Its effect on the amplitudes of the P-DMR events induced by (a) thrombin (40 unit/ml) or (b) trypsin (200 nM) was analyzed. In comparison, the effect of αCD was also included. (c) The DMR signals of A431 cells induced by A23187 without or with the pre-treatment with 5 mM mβCD.
Fang and Ferrie BMC Cell Biology 2007 8:24 doi:10.1186/1471-2121-8-24